modified eagle medium dmem high glucose medium Search Results


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FIGURE 7 25HC suppresses HPV infection by restricting small GTPase prenylation. (A) Pan‐prenylation level was suppressed by 25HC. (B) Membrane proteins were collected for Western blot. Membrane‐bound, prenylated Rac1, Cdc42, RhoA and Rap1 expressions were reduced by 25HC. Na+/K+ ATPase (ATP1a1) was used as membrane loading control. (C) The prenylation inhibitors (a) GGTI‐298 and (b) FTI‐277 revealed anti‐HPV16 PsV effect in inhibitory assays. GGTI‐298 and FTI‐277 were serially diluted (0−10 μM) in <t>DMEM</t> and incubated with HPV16 PsV. Mixtures were added to cells and incubated for 16 h for inhibition assay. (D) Farnesylation substrate FPP rescued anti‐HPV capability of 25HC. FPP (10 μM) were mixed with serially diluted 25HC, and added to cells with HPV16 PsV. (E) Prenylation inhibitor GGTI‐298 inhibited LIMK1/cofilin phosphorylation.
High Glucose Dulbecco S Modified Eagle S Medium Dmem, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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FIGURE 7 25HC suppresses HPV infection by restricting small GTPase prenylation. (A) Pan‐prenylation level was suppressed by 25HC. (B) Membrane proteins were collected for Western blot. Membrane‐bound, prenylated Rac1, Cdc42, RhoA and Rap1 expressions were reduced by 25HC. Na+/K+ ATPase (ATP1a1) was used as membrane loading control. (C) The prenylation inhibitors (a) GGTI‐298 and (b) FTI‐277 revealed anti‐HPV16 PsV effect in inhibitory assays. GGTI‐298 and FTI‐277 were serially diluted (0−10 μM) in <t>DMEM</t> and incubated with HPV16 PsV. Mixtures were added to cells and incubated for 16 h for inhibition assay. (D) Farnesylation substrate FPP rescued anti‐HPV capability of 25HC. FPP (10 μM) were mixed with serially diluted 25HC, and added to cells with HPV16 PsV. (E) Prenylation inhibitor GGTI‐298 inhibited LIMK1/cofilin phosphorylation.
Dulbecco S Modified Eagle S Medium Dmem, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Beijing Neuronbc dulbecco's modified eagle's medium (dmem)
FIGURE 7 25HC suppresses HPV infection by restricting small GTPase prenylation. (A) Pan‐prenylation level was suppressed by 25HC. (B) Membrane proteins were collected for Western blot. Membrane‐bound, prenylated Rac1, Cdc42, RhoA and Rap1 expressions were reduced by 25HC. Na+/K+ ATPase (ATP1a1) was used as membrane loading control. (C) The prenylation inhibitors (a) GGTI‐298 and (b) FTI‐277 revealed anti‐HPV16 PsV effect in inhibitory assays. GGTI‐298 and FTI‐277 were serially diluted (0−10 μM) in <t>DMEM</t> and incubated with HPV16 PsV. Mixtures were added to cells and incubated for 16 h for inhibition assay. (D) Farnesylation substrate FPP rescued anti‐HPV capability of 25HC. FPP (10 μM) were mixed with serially diluted 25HC, and added to cells with HPV16 PsV. (E) Prenylation inhibitor GGTI‐298 inhibited LIMK1/cofilin phosphorylation.
Dulbecco's Modified Eagle's Medium (Dmem), supplied by Beijing Neuronbc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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BioWhittaker Molecular Applications dulbecco’s modified eagle’s medium with high glucose
FIGURE 7 25HC suppresses HPV infection by restricting small GTPase prenylation. (A) Pan‐prenylation level was suppressed by 25HC. (B) Membrane proteins were collected for Western blot. Membrane‐bound, prenylated Rac1, Cdc42, RhoA and Rap1 expressions were reduced by 25HC. Na+/K+ ATPase (ATP1a1) was used as membrane loading control. (C) The prenylation inhibitors (a) GGTI‐298 and (b) FTI‐277 revealed anti‐HPV16 PsV effect in inhibitory assays. GGTI‐298 and FTI‐277 were serially diluted (0−10 μM) in <t>DMEM</t> and incubated with HPV16 PsV. Mixtures were added to cells and incubated for 16 h for inhibition assay. (D) Farnesylation substrate FPP rescued anti‐HPV capability of 25HC. FPP (10 μM) were mixed with serially diluted 25HC, and added to cells with HPV16 PsV. (E) Prenylation inhibitor GGTI‐298 inhibited LIMK1/cofilin phosphorylation.
Dulbecco’s Modified Eagle’s Medium With High Glucose, supplied by BioWhittaker Molecular Applications, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Biochrom high-glucose [4.5 g/l] dulbecco’s modified eagle’s medium including stable glutamine (dmem)
FIGURE 7 25HC suppresses HPV infection by restricting small GTPase prenylation. (A) Pan‐prenylation level was suppressed by 25HC. (B) Membrane proteins were collected for Western blot. Membrane‐bound, prenylated Rac1, Cdc42, RhoA and Rap1 expressions were reduced by 25HC. Na+/K+ ATPase (ATP1a1) was used as membrane loading control. (C) The prenylation inhibitors (a) GGTI‐298 and (b) FTI‐277 revealed anti‐HPV16 PsV effect in inhibitory assays. GGTI‐298 and FTI‐277 were serially diluted (0−10 μM) in <t>DMEM</t> and incubated with HPV16 PsV. Mixtures were added to cells and incubated for 16 h for inhibition assay. (D) Farnesylation substrate FPP rescued anti‐HPV capability of 25HC. FPP (10 μM) were mixed with serially diluted 25HC, and added to cells with HPV16 PsV. (E) Prenylation inhibitor GGTI‐298 inhibited LIMK1/cofilin phosphorylation.
High Glucose [4.5 G/L] Dulbecco’s Modified Eagle’s Medium Including Stable Glutamine (Dmem), supplied by Biochrom, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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Dominique DUTSCHER SAS dulbecco´s modified eagle´s medium (dmem-high glucose
FIGURE 7 25HC suppresses HPV infection by restricting small GTPase prenylation. (A) Pan‐prenylation level was suppressed by 25HC. (B) Membrane proteins were collected for Western blot. Membrane‐bound, prenylated Rac1, Cdc42, RhoA and Rap1 expressions were reduced by 25HC. Na+/K+ ATPase (ATP1a1) was used as membrane loading control. (C) The prenylation inhibitors (a) GGTI‐298 and (b) FTI‐277 revealed anti‐HPV16 PsV effect in inhibitory assays. GGTI‐298 and FTI‐277 were serially diluted (0−10 μM) in <t>DMEM</t> and incubated with HPV16 PsV. Mixtures were added to cells and incubated for 16 h for inhibition assay. (D) Farnesylation substrate FPP rescued anti‐HPV capability of 25HC. FPP (10 μM) were mixed with serially diluted 25HC, and added to cells with HPV16 PsV. (E) Prenylation inhibitor GGTI‐298 inhibited LIMK1/cofilin phosphorylation.
Dulbecco´S Modified Eagle´S Medium (Dmem High Glucose, supplied by Dominique DUTSCHER SAS, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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FUJIFILM dulbecco’s modified eagle medium, high glucose
FIGURE 7 25HC suppresses HPV infection by restricting small GTPase prenylation. (A) Pan‐prenylation level was suppressed by 25HC. (B) Membrane proteins were collected for Western blot. Membrane‐bound, prenylated Rac1, Cdc42, RhoA and Rap1 expressions were reduced by 25HC. Na+/K+ ATPase (ATP1a1) was used as membrane loading control. (C) The prenylation inhibitors (a) GGTI‐298 and (b) FTI‐277 revealed anti‐HPV16 PsV effect in inhibitory assays. GGTI‐298 and FTI‐277 were serially diluted (0−10 μM) in <t>DMEM</t> and incubated with HPV16 PsV. Mixtures were added to cells and incubated for 16 h for inhibition assay. (D) Farnesylation substrate FPP rescued anti‐HPV capability of 25HC. FPP (10 μM) were mixed with serially diluted 25HC, and added to cells with HPV16 PsV. (E) Prenylation inhibitor GGTI‐298 inhibited LIMK1/cofilin phosphorylation.
Dulbecco’s Modified Eagle Medium, High Glucose, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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EuroClone dmem low glucose ecm007l
FIGURE 7 25HC suppresses HPV infection by restricting small GTPase prenylation. (A) Pan‐prenylation level was suppressed by 25HC. (B) Membrane proteins were collected for Western blot. Membrane‐bound, prenylated Rac1, Cdc42, RhoA and Rap1 expressions were reduced by 25HC. Na+/K+ ATPase (ATP1a1) was used as membrane loading control. (C) The prenylation inhibitors (a) GGTI‐298 and (b) FTI‐277 revealed anti‐HPV16 PsV effect in inhibitory assays. GGTI‐298 and FTI‐277 were serially diluted (0−10 μM) in <t>DMEM</t> and incubated with HPV16 PsV. Mixtures were added to cells and incubated for 16 h for inhibition assay. (D) Farnesylation substrate FPP rescued anti‐HPV capability of 25HC. FPP (10 μM) were mixed with serially diluted 25HC, and added to cells with HPV16 PsV. (E) Prenylation inhibitor GGTI‐298 inhibited LIMK1/cofilin phosphorylation.
Dmem Low Glucose Ecm007l, supplied by EuroClone, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Biochrom dulbecco’s modified eagle’s medium with 3.7 g/l sodium bicarbonate
FIGURE 7 25HC suppresses HPV infection by restricting small GTPase prenylation. (A) Pan‐prenylation level was suppressed by 25HC. (B) Membrane proteins were collected for Western blot. Membrane‐bound, prenylated Rac1, Cdc42, RhoA and Rap1 expressions were reduced by 25HC. Na+/K+ ATPase (ATP1a1) was used as membrane loading control. (C) The prenylation inhibitors (a) GGTI‐298 and (b) FTI‐277 revealed anti‐HPV16 PsV effect in inhibitory assays. GGTI‐298 and FTI‐277 were serially diluted (0−10 μM) in <t>DMEM</t> and incubated with HPV16 PsV. Mixtures were added to cells and incubated for 16 h for inhibition assay. (D) Farnesylation substrate FPP rescued anti‐HPV capability of 25HC. FPP (10 μM) were mixed with serially diluted 25HC, and added to cells with HPV16 PsV. (E) Prenylation inhibitor GGTI‐298 inhibited LIMK1/cofilin phosphorylation.
Dulbecco’s Modified Eagle’s Medium With 3.7 G/L Sodium Bicarbonate, supplied by Biochrom, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Merck KGaA high-glucose dulbecco's modified eagle medium (dmem)
FIGURE 7 25HC suppresses HPV infection by restricting small GTPase prenylation. (A) Pan‐prenylation level was suppressed by 25HC. (B) Membrane proteins were collected for Western blot. Membrane‐bound, prenylated Rac1, Cdc42, RhoA and Rap1 expressions were reduced by 25HC. Na+/K+ ATPase (ATP1a1) was used as membrane loading control. (C) The prenylation inhibitors (a) GGTI‐298 and (b) FTI‐277 revealed anti‐HPV16 PsV effect in inhibitory assays. GGTI‐298 and FTI‐277 were serially diluted (0−10 μM) in <t>DMEM</t> and incubated with HPV16 PsV. Mixtures were added to cells and incubated for 16 h for inhibition assay. (D) Farnesylation substrate FPP rescued anti‐HPV capability of 25HC. FPP (10 μM) were mixed with serially diluted 25HC, and added to cells with HPV16 PsV. (E) Prenylation inhibitor GGTI‐298 inhibited LIMK1/cofilin phosphorylation.
High Glucose Dulbecco's Modified Eagle Medium (Dmem), supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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FIGURE 7 25HC suppresses HPV infection by restricting small GTPase prenylation. (A) Pan‐prenylation level was suppressed by 25HC. (B) Membrane proteins were collected for Western blot. Membrane‐bound, prenylated Rac1, Cdc42, RhoA and Rap1 expressions were reduced by 25HC. Na+/K+ ATPase (ATP1a1) was used as membrane loading control. (C) The prenylation inhibitors (a) GGTI‐298 and (b) FTI‐277 revealed anti‐HPV16 PsV effect in inhibitory assays. GGTI‐298 and FTI‐277 were serially diluted (0−10 μM) in DMEM and incubated with HPV16 PsV. Mixtures were added to cells and incubated for 16 h for inhibition assay. (D) Farnesylation substrate FPP rescued anti‐HPV capability of 25HC. FPP (10 μM) were mixed with serially diluted 25HC, and added to cells with HPV16 PsV. (E) Prenylation inhibitor GGTI‐298 inhibited LIMK1/cofilin phosphorylation.

Journal: Journal of medical virology

Article Title: 25-hydroxycholesterol inhibits human papillomavirus infection in cervical epithelial cells by perturbing cytoskeletal remodeling.

doi: 10.1002/jmv.28834

Figure Lengend Snippet: FIGURE 7 25HC suppresses HPV infection by restricting small GTPase prenylation. (A) Pan‐prenylation level was suppressed by 25HC. (B) Membrane proteins were collected for Western blot. Membrane‐bound, prenylated Rac1, Cdc42, RhoA and Rap1 expressions were reduced by 25HC. Na+/K+ ATPase (ATP1a1) was used as membrane loading control. (C) The prenylation inhibitors (a) GGTI‐298 and (b) FTI‐277 revealed anti‐HPV16 PsV effect in inhibitory assays. GGTI‐298 and FTI‐277 were serially diluted (0−10 μM) in DMEM and incubated with HPV16 PsV. Mixtures were added to cells and incubated for 16 h for inhibition assay. (D) Farnesylation substrate FPP rescued anti‐HPV capability of 25HC. FPP (10 μM) were mixed with serially diluted 25HC, and added to cells with HPV16 PsV. (E) Prenylation inhibitor GGTI‐298 inhibited LIMK1/cofilin phosphorylation.

Article Snippet: Cells were cultured in high‐ glucose Dulbecco's modified Eagle's medium (DMEM) (HyClone) supplemented with 10% fetal bovine serum (FBS) (Yeasen), and incubated in a humidified environment containing 5% CO2 at 37°C.

Techniques: Infection, Membrane, Western Blot, Control, Incubation, Inhibition, Phospho-proteomics